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BIOL484

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Advanced DNA Techniques

Biology & ChemistryUndergraduateCollege of Science and Engineering

Course Outline

Nucleic acid metabolism and its relationship to DNA sequencing. 10% Eukaryotic and prokaryotic model genetic systems of gene regulation. 10% Polymerase chain reaction and its variants. 10% Plasmid and genomic DNA purification. 15% DNA cloning via restriction enzyme mapping, recombinational cloning, and virtual cloning. 15% Genetic engineering via CRISPR/Cas9. 15% Bioinformatic database access. 10% Annotation of nucleic acid and amino acid metabolic pathways. 15%